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Polyembryonic seedlings - how do you really know?

 
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klemmd
Citruholic
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Joined: 23 Jan 2013
Posts: 55
Location: Annandale, VA

Posted: Wed 04 Dec, 2013 12:39 pm

I am starting some citrandarins from seed. I've read that with this particular citrus hybrid at least 50% of its seedlings could be zygotic.

So if I want clones, as I observe its seedlings, shall I assume I should throw out all the seedlings that are single plants -- those that have only one sprout per seed?

And shall I keep only the seeds that are putting out multiple sprouts per seed?

But it seems to me I can't be assured that one of the multiple sprouts isn't zygotic. One of them MIGHT be a zygotic plant, correct?

So now my question is, what is the best way to tell if any of the sprouts are zygotic?

Should I be able to tell by comparing plant size and vigor? Or do I wait until plants get larger and look for other differences like leaf shape? What if leaf shape differences are so subtle that one can't tell?

Are there any other ways to tell?

Or is one NEVER completely sure without doing a DNA test of some kind or waiting until it has borne fruit???

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Laaz
Site Owner
Site Owner


Joined: 12 Nov 2005
Posts: 5642
Location: Dorchester County, South Carolina

Posted: Wed 04 Dec, 2013 2:59 pm

What I have found is that most hybrid citrus does come true.

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ilyaC
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Joined: 04 Sep 2009
Posts: 274
Location: France, 40km South of Paris

Posted: Wed 04 Dec, 2013 6:49 pm

klemmd wrote:

So now my question is, what is the best way to tell if any of the sprouts are zygotic?

Sometimes it is quite obvious. Below is zygotic citrangor (poncirusXAmoa8) sprouting from polyembryonic poncirus seed.



After separation:


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klemmd
Citruholic
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Joined: 23 Jan 2013
Posts: 55
Location: Annandale, VA

Posted: Wed 04 Dec, 2013 11:51 pm

Laaz wrote:
What I have found is that most hybrid citrus does come true.


But not citrandarin. it is approximately 50% zygotic, according to Dept of Ag in Florida.

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klemmd
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Joined: 23 Jan 2013
Posts: 55
Location: Annandale, VA

Posted: Wed 04 Dec, 2013 11:52 pm

ilyaC

Thanks for posting photos of a good example!

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pagnr
Citrus Guru
Citrus Guru


Joined: 23 Aug 2008
Posts: 407
Location: Australia

Posted: Thu 05 Dec, 2013 11:01 pm

All you can do is grow extra than what you think you will need, if the rate of off types is 50%, you will need to plant twice as many seed as your required rootstocks. With Citranges + Citrumelos etc, the variation will be anything from more more Trifoliata like to more Citrus like. more vigorous to less vigorous.
In the middle will be a fairly uniform type, about same height, thickness, leaf shape, thorn length etc, which will be suitable as rootstocks.
You probably need the plants to be 6 to 12 inches tall to grade them, although some will stand out before.
Any off types that are equally vigorous, I would use anyway.
Any interesting types I would keep, especially single leaf 2nd gen Citranges.
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