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grafting seeds?

 
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Descender



Joined: 07 Apr 2013
Posts: 6
Location: Fresno, Ca

Posted: Tue 23 Apr, 2013 7:35 pm

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Millet
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Joined: 13 Nov 2005
Posts: 6657
Location: Colorado

Posted: Wed 24 Apr, 2013 12:33 am

Strange indeed. - Millet
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Descender



Joined: 07 Apr 2013
Posts: 6
Location: Fresno, Ca

Posted: Wed 24 Apr, 2013 2:45 am

How would that even work? Pull the embryos out of a seed and jam it in a rootstock t bud style?
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Fascist Nation
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Joined: 23 Dec 2011
Posts: 26
Location: Phoenix, AZ 9b, Sunset 13, AHS 11

Posted: Sat 27 Apr, 2013 5:51 pm

Wow, I might pay to get the paper on that one. (Of course as a taxpayer I was held up to pay for the research).

I can see the utility in curing some cultivars of diseases that are not transmitted via seed but are present in all (most) representatives of that cultivar.

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ilyaC
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Joined: 04 Sep 2009
Posts: 274
Location: France, 40km South of Paris

Posted: Wed 22 May, 2013 12:15 pm

It is very simple: seeds are washed, seed coat is removed, longitudinal incision is made by the scalpel on cotyledons to insure the contact with rootstock .
The rest resembles bud grafting.


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Laaz
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Joined: 12 Nov 2005
Posts: 5642
Location: Dorchester County, South Carolina

Posted: Wed 22 May, 2013 12:17 pm

But what is the purpose? It will still be a juvenile plant...

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ilyaC
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Joined: 04 Sep 2009
Posts: 274
Location: France, 40km South of Paris

Posted: Wed 22 May, 2013 1:03 pm

They list a rapid growth of "seedlings" for hybrid selection and pathogen resistance studies.

" ...the seed grafting method has potential
applications in fruit tree breeding when the female
parent in a cross is mono-embryonic. In such situations,
hybrid germination, early growth and tree establishment
could be speeded-up considerably using the seed grafting
technique. In addition, this method could facilitate
screening and selection for resistance to fruit tree
diseases, in particular those caused by graft-transmissible
systemic pathogens. It could also facilitate selection
among seeds derived from different sources, including
seeds of extremely variable genetic backgrounds
obtained by crossing members of distant genera, or seeds
from variants of established varieties derived from
treatments with chemical mutagens or radiation.
Grafting seeds directly onto systemically-infected
rootstock plants would facilitate the rapid preliminary
evaluation and selection of grafted sprouts that appeared
normal and healthy, which may indicate resistance (or
tolerance) to a particular pathogen."

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Laaz
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Joined: 12 Nov 2005
Posts: 5642
Location: Dorchester County, South Carolina

Posted: Wed 22 May, 2013 1:14 pm

Thanks Ilya.

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Sylvain
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Joined: 16 Nov 2007
Posts: 790
Location: Bergerac, France.

Posted: Wed 22 May, 2013 3:22 pm

Very interesting!!!
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ilyaC
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Joined: 04 Sep 2009
Posts: 274
Location: France, 40km South of Paris

Posted: Wed 22 May, 2013 4:28 pm

As an example what can be achieved in 10 months with these "seedlings".



"Two 10-month-old ‘Minneola’ tangelo shoots (arrowed) derived from seeds grafted onto a ‘Troyer’ citrange seedling (Panel A). Arrows indicate the
two ‘Minneola’ shoots at the points of the grafts. A 10-month-old field-grown ‘Shamouti’ sweet orange tree grown from a seed grafted onto a ‘Troyer’
citrange seedling (Panels B, C). The circles encompass the region of the graft union, which is seen in a closer view in Panel C. The original ‘Troyer’
rootstock is the lower (darker) portion and the white atrophied “shoot” (“stick”)."

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GregMartin
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Joined: 12 Jan 2011
Posts: 265
Location: southern Maine, zone 5/6

Posted: Wed 22 May, 2013 9:46 pm

Wow, thanks Ilya!
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